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  • 進口和國產色譜柱|菲羅門色譜柱|手性柱|氣相柱|SPE|高效液相色譜柱
    • ACE Preparative HPLC Columns Chromatographers with experience in preparative HPLC know that resolution and loadability are of the utmost importance.

      The greater the resolution, the higher the sample load and the faster pure compound is obtained. The ability to optimize resolution at the preparative scale means starting with high performance separations at the analytical scale.

      The same features that make ACE ultra-inert base deactivated analytical columns the choice of method development chemists also make them the ideal choice for scale-up and process methods.
    詳情

    ACE Preparative HPLC Columns


    ■  Ultra high purity base deactivated silica
    ■  5, 10 and 15μm particle sizes available
    ■  Fully validated columns
    ■  Exceptional reproducibility
    ■  Excellent efficiencies
    ■  High sample recovery
    ■  Excellent column lifetime
    ■  100? and 300? pore sizes

    Get High Purity Product Fast


    ACE preparative HPLC columns are available in a wide range of column dimensions and particle sizes for complete optimization of  preparative assays.

    For maximum loadability, 30mm or 50mm i.d. columns are recommended. Use a 50mm length ‘combinatorial’ column with a 5μm particle size to maximize the speed of your analysis.

    To maximize resolution, choose a 250mm length column with a 5μm particle size.

    Loading specifications.

    Column size Column id (mm) Relative flow rate (ml/min) Weight of phase (g) Typical injection volume (μl) Sample capacity per injection
              Optimum Overload
    Analytical 4.6 1.0 2.5 10 2mg 85mg
    Semi-preparative 7.75 2.8 7 30 6mg 240mg
    Semi-preparative 10 4.7 12 50 10mg 400mg
    Preparative 21.2 21 53 200 45mg 1.8g
    Preparative 30 42.5 106 400 90mg 3.6g
    Process 50 118 295 1200 250mg 10g
    Process 100 473 1182 4800 1g 40g


    The following figure demonstrates a typical preparative method development strategy. Since ACE columns are shown to give reproducible scale-up and are available in the widest range of column dimensions, loading studies may be performed at an analytical level with complete confidence that this can be fully transferred to preparative scale columns.

     

    ACE preparative HPLC columns offer the following benefits:


    ■  Loadability – high surface area and carbon load for maximum sample capacity


    ■  Selectivity – available in 8 surface chemistries to optimize sample capacity


    ■  Rugged – reliable, long-term performance


    ■  Guaranteed reproducibility – complete column/batch validation as for ACE analytical columns


    ■  Guard cartridges – available for maximum column protection

    Increased Preparative Column Lifetimes

    A fundamental requirement for successful preparative applications is a robust, rugged column. ACE materials have previously been shown to have excellent chemical stability under highly acidic  and highly basic  conditions. The high mechanical stability of the ultra-inert ACE silica and the unique preparative packing methods employed result in a high efficiency, extremely stable packed silica bed – leading to increased preparative column lifetimes. The following figure demonstrates the excellent column performance maintained with a 50 x 21.2mm ACE 5 AQ column over an extended test period.

     Catalogue >

     

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    中文有码人妻字幕在线
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