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ACE Preparative HPLC Columns
Chromatographers with experience in preparative HPLC know that resolution and loadability are of the utmost importance.
The greater the resolution, the higher the sample load and the faster pure compound is obtained. The ability to optimize resolution at the preparative scale means starting with high performance separations at the analytical scale.
The same features that make ACE ultra-inert base deactivated analytical columns the choice of method development chemists also make them the ideal choice for scale-up and process methods.
ACE Preparative HPLC Columns
■ Ultra high purity base deactivated silica
■ 5, 10 and 15μm particle sizes available
■ Fully validated columns
■ Exceptional reproducibility
■ Excellent efficiencies
■ High sample recovery
■ Excellent column lifetime
■ 100? and 300? pore sizes
Get High Purity Product Fast
ACE preparative HPLC columns are available in a wide range of column dimensions and particle sizes for complete optimization of preparative assays.
For maximum loadability, 30mm or 50mm i.d. columns are recommended. Use a 50mm length ‘combinatorial’ column with a 5μm particle size to maximize the speed of your analysis.
To maximize resolution, choose a 250mm length column with a 5μm particle size.
Loading specifications.
| Column size | Column id (mm) | Relative flow rate (ml/min) | Weight of phase (g) | Typical injection volume (μl) | Sample capacity per injection | |
| Optimum | Overload | |||||
| Analytical | 4.6 | 1.0 | 2.5 | 10 | 2mg | 85mg |
| Semi-preparative | 7.75 | 2.8 | 7 | 30 | 6mg | 240mg |
| Semi-preparative | 10 | 4.7 | 12 | 50 | 10mg | 400mg |
| Preparative | 21.2 | 21 | 53 | 200 | 45mg | 1.8g |
| Preparative | 30 | 42.5 | 106 | 400 | 90mg | 3.6g |
| Process | 50 | 118 | 295 | 1200 | 250mg | 10g |
| Process | 100 | 473 | 1182 | 4800 | 1g | 40g |
The following figure demonstrates a typical preparative method development strategy. Since ACE columns are shown to give reproducible scale-up and are available in the widest range of column dimensions, loading studies may be performed at an analytical level with complete confidence that this can be fully transferred to preparative scale columns.
ACE preparative HPLC columns offer the following benefits:
■ Loadability – high surface area and carbon load for maximum sample capacity
■ Selectivity – available in 8 surface chemistries to optimize sample capacity
■ Rugged – reliable, long-term performance
■ Guaranteed reproducibility – complete column/batch validation as for ACE analytical columns
■ Guard cartridges – available for maximum column protection
Increased Preparative Column Lifetimes
A fundamental requirement for successful preparative applications is a robust, rugged column. ACE materials have previously been shown to have excellent chemical stability under highly acidic and highly basic conditions. The high mechanical stability of the ultra-inert ACE silica and the unique preparative packing methods employed result in a high efficiency, extremely stable packed silica bed – leading to increased preparative column lifetimes. The following figure demonstrates the excellent column performance maintained with a 50 x 21.2mm ACE 5 AQ column over an extended test period.
