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  • 進口和國產色譜柱|菲羅門色譜柱|手性柱|氣相柱|SPE|高效液相色譜柱
    • ACE 300? HPLC columns ACE 300? columns are available in an extensive range of dimensions and particle sizes for use in micro-scale separations, LC/MS analyses, high speed preparative analyses up to process scale.
    詳情

    ACE 300? HPLC columns

    Excellent peak shape and reproducibility have established ACE HPLC columns as the finest available. This quality is now available for protein chemists desiring the  utmost in performance and reproducibility for the separation of peptides, proteins and other high molecular weight biomolecules.

     

    ACE 300? columns are available in an extensive range of dimensions and particle sizes for use in micro-scale separations, LC/MS analyses, high speed preparative analyses up to process scale.



    ACE 300? Columns for Biotechnology

    ■ 300? ultra high purity silica
    ■ Ultimate protein and peptide application column
    ■ C18, C8, C4, CN and Phenyl chemistries
    ■ 3μm, 5μm and 10μm particle sizes
    ■ Unmatched reproducibility
    ■ Exceptional chemical stability

    Phase Functional group Particle size (μm) Pore size (?) Surface area (m2/g) Endcapped Carbon load (%)
    C18-300 Octadecyl 3,5,10 300 100 yes 9.0
    C8-300 Octyl 3,5,10 300 100 yes 5.0
    C4-300 Butyl 3,5,10 300 100 yes 2.6
    CN-300 Cyano 3,5,10 300 100 yes 2.6
    Ph-300 Phenyl 3,5,10 300 100 yes 5.3

    Comparison of Leading 300? 5μm C18 Columns

    In order to demonstrate the benefits of ultra-inert phases in biomolecule analysis, several commercially available 300? pore size reversed-phase columns were  tested using three different samples:

    • neutral molecules to measure efficiency,
    • pyridine/phenol to measure silanol activity and
    • antidepressants to measure both  silanol activity and metal content

     

    These are the same test procedures typically used to evaluate standard pore size columns (eg 100?) used for the analysis of small  molecules in the chemical and pharmaceutical industries. Columns were ranked by efficiency, N, measured at 10% peak height. In addition to measuring overall efficiency, this value also takes into consideration peak tailing usually caused by silanol interactions. The table below summarises the performance of various columns as determined by each test along with an overall ranking based on a combination of all three tests.

    ACE 300? Columns for Peptide and Protein Analyses

    The chromatography of biomolecules, in particular peptides and proteins, can be improved by using HPLC columns packed with ultra-inert stationary phases. These columns will have reduced levels of silanol and metal activity to interfere with the separation. In addition, ultra-inert stationary phases perform well even when  using low levels of TFA in the mobile phase. Using reduced levels of TFA improves mass spectral detection, in addition to providing a means of increasing selectivity  and resolution.


    Mobile phase pH is another powerful means for improving selectivity and resolution. Ultra-inert columns, such as ACE, show no loss in performance at higher pH.  Methods developed on ultra-inert columns will be more rugged over time as these columns are more reproducible column-to-column and lot-to-lot.

    ACE 300? Columns for Peptide and Protein Analyses

    Chromatographers prefer inert stationary phases for the reversed-phase HPLC of ionic compounds because they minimize the negative effect of silanols on the  separation. This results in improved peak shape and reproducibility when separating compounds that contain polar functional groups, especially amines.

    A new  generation of ultra-inert stationary phases, with extremely low silanol activity, has made it possible to achieve even better peak shape and reproducibility when  separating these types of compounds. Scientists working with small molecules have been rapidly adopting this new technology and the recent introduction of  wide-pore (300?) ultra-inert phases makes the benefits of this technology available to those wanting to separate peptides and proteins by reversed-phase HPLC.

     

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