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    • ACE Excel GLYCAN Columns UHPLC/HPLC columns for reproducible glycan analysis
    詳情

    AVANTOR® ACE® EXCEL® GLYCAN

    GLYCAN ANALYSIS
    ? Analysis of the glycosylation pattern of biopharmaceutical compounds is essential for their complete characterisation
    ? Monoclonal antibodies (mAbs) are one of the largest growing areas of biotherapeutic interest and are heavily glycosylated molecules with each mAb containing at least two glycosylation sites as shown in Figure 1 below
    ? Changes to the glycosylation of a biopharmaceutical can affect the efficacy and safety of the drug, so it is extremely important that it is thoroughly monitored
    ? Due to the highly polar nature of released glycans, a hydrophilic stationary phase is ideal for their separation
    ? Labelling with a fluorophore such as 2-aminobenzamide (2-AB) allows the released glycans to be analysed using fluorescence detection

    Figure 1: Schematic showing glycosylation regions of a monoclonal antibody.

    To extend column lifetime ACE UHPLC guard cartridges (Cat. No. EXL-1P16-GD2U / Cat. No. EXL-1P16-GD4U) are recommended.
    For the guard system, guard cartridge holder (H0011) is also required.
    For HPLC column connections up to 6000 psi, PEEK? fingertight fittings (Cat. No. ACE-CC10) are recommended - for further details please contact 020-22826668
    For UHPLC column connections up to 25000 psi, reuseable fittings (Cat. No. EXL-CC10) are recommended - for further details please contact 020-22826668

     

    GUARANTEED REPRODUCIBILITY
    ? The ACE® Excel® Glycan column provides excellent batch-to-batch reproducibility as shown below
    ? The dextran ladder standard consists of a mixture of glucose homopolymers of between 1 to 15 monomer units, and is a useful tool in identifying more complex glycoforms based upon correlated retention times

     

    BATCH-TO-BATCH REPRODUCIBILITY

    Sample: 2-AB labelled Dextran Ladder standard
    Column: ACE Excel 1.7 Glycan, 100x2.1 mm
    Cat. No.: EXL-1716-1002
    Mobile phase: A = 100 mM ammonium formate (pH 4.5) and B = MeCN
    Gradient: 75 – 52.5% B in 24 min
    Flow rate: 0.5 ml/min
    Temperature: 55 °C
    Injection: 1 μl
    Detection: FLD, λex = 260 nm, λem = 430 nm

     

    GUARANTEED SCALABILITY

    The ACE® Excel® Glycan column provides exceptionally reproducible scalability between the 1.7 μm and 3 μm particle sizes, as demonstrated below

    PARTICLE SIZE COMPARISON

    Sample: 2-AB labelled Dextran Ladder standard
    Columns: ACE Excel 1.7 Glycan, 100x2.1 mm; ACE Excel 3 Glycan, 100x2.1 mm
    Cat. No.: EXL-1716-1002 and EXL-1116-1002
    Mobile phase: A = 100 mM ammonium formate (pH 4.5) and B = MeCN
    Gradient: 75 – 52.5% B in 24 min
    Flow rate: 0.5 ml/min
    Temperature: 55 °C
    Injection: 1 μl
    Detection: FLD, λex = 260 nm, λem = 430 nm

     

    EXCELLENT COLUMN STABILITY
    ? The ACE® Excel® Glycan column shows good stability for glycan analysis over several thousand column volumes as seen in the graph below

     

    COLUMN STABILITY UNDER GLYCAN ANALYSIS CONDITIONS

    Sample: 2-AB labelled Dextran Ladder standard
    Column: ACE Excel 1.7 Glycan, 50x2.1 mm
    Cat. No.: EXL-1716-0502
    Mobile phase: A = 100 mM ammonium formate (pH 4.5) and B = MeCN
    Gradient: 75 – 60% B in 8 min
    Flow rate: 0.5 ml/min
    Temperature: 55 °C
    Injection: 0.5 μl
    Detection: FLD, λex = 260 nm, λem = 430 nm

     

    AVANTOR® ACE® EXCEL® GLYCAN UHPLC/HPLC ORDERING INFORMATION

     

     

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