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    • ACE Excel Oligo U/HPLC Columns UHPLC / HPLC COLUMNS FOR REPRODUCIBLE OLIGONUCLEOTIDE ANALYSIS
    詳情

    AVANTOR® ACE® EXCEL® OLIGO COLUMN OLIGONUCLEOTIDE ANALYSIS
    ? Oligonucleotides are being synthesised for therapeutic usage against many diseases including genetic disorders and cancer
    ? Several impurities can be produced during oligonucleotide synthesis (e.g.failure sequences) which need to be separated from the desired product
    ? Ion-pair RPLC can be employed for the analysis of trityl-off deprotectedoligonucleotides
    ? Triethylammonium acetate (TEAA) is used to ion pair with the negativelycharged phosphodiester backbone of the oligonucleotide
    ? The stationary phase is chemically modified to withstand an extended pH range


    OLIGONUCLEOTIDE LADDER STANDARD
    ? The oligonucleotide ladder standard demonstrated in this brochure consists of six DNA oligonucleotides of varying sequence length from 15 to 40 mer
    ? Each oligonucleotide sequence is made up of several thymidine monomers joined together via phosphodiester linkages as shown below

    PRODUCT SPECIFICATIONS

       


    To extend column lifetime ACE UHPLC guard cartridges (EXL-1P15-GD2U / EXL-1P15-GD4U) are recommended. For the guard system, a guard cartridge holder (H0011) is also required.
    For HPLC column connections up to 6000 psi, PEEK? fingertight fittings (ACE-CC10) are recommended - for further details please contact 020-22826668
    For UHPLC column connections up to 25000 psi, reuseable fittings (EXL-CC10) are recommended - for further details please contact 020-22826668

    GUARANTEED REPRODUCIBILITY
    ? The ACE Excel Oligo column provides excellent batch-to-batch reproducibility as shown below

    BATCH-TO-BATCH REPRODUCIBILITY

     

    Columns: ACE Excel 1.7 Oligo, 50x2.1 mm
    Cat. No.: EXL-1715-0502
    Mobile phase A: 80 mM TEAA in H2O (pH 7.0)
    Mobile phase B: 80 mM TEAA in MeCN
    Gradient: 12 – 15% B in 10 min
    Flow rate: 0.2 ml/min
    Temperature: 60 °C
    Injection: 5 μl
    Detection: 260 nm

    Columns: ACE Excel 3 Oligo, 150x4.6 mm
    Cat. No.: EXL-1115-1546
    Mobile phase A: 80 mM TEAA in H2O (pH 7.0)
    Mobile phase B: 80 mM TEAA in MeCN
    Gradient: 12 – 15% B in 30 min
    Flow rate: 1.0 ml/min
    Temperature: 60 °C
    Injection: 20 μl
    Detection: 260 nm

    MASS-SPEC COMPATIBLE
    The ACE Oligo column is compatible with typical MS method conditions for oligonucleotide analysis
    HFIP is commonly used in the mobile phase and was used for the separation shown below, in combination with dibutylamine

    Sample: Oligonucleotide ladder standard
    Column: ACE Excel 1.7 Oligo, 100x2.1 mm
    Cat. No.: EXL-1715-1002
    Mobile phase A: 15 mM dibutylamine + 25 mM 1,1,1,3,3,3-hexafluoropropan-2-ol in H2O
    Mobile phase B: 15 mM dibutylamine + 25 mM 1,1,1,3,3,3-hexafluoropropan-2-ol in MeOH
    Gradient: 30 – 50% B in 15 min
    Flow rate: 0.6 ml/min
    Temperature: 60 °C
    Injection: 10 μl
    Detection: 260 nm

     

    EXCELLENT COLUMN STABILITY
    ? The ACE Oligo column shows excellent stability for oligonucleotide analysis over 5000 column volumes as shown below

     

    COLUMN STABILITY STUDY

    Sample: Oligonucleotide ladder standard
    Mobile phase A: 100 mM TEAA in H2O (pH 7.0)
    Mobile phase B: 100 mM TEAA in MeCN (pH 7.0)
    Gradient: 10 – 14% B in 10 min
    Flow rate: 0.6 ml/min
    Temperature: 65 °C
    Injection: 5 μl
    Detection: 260 nm

     

    ORDERING INFORMATION
    U/HPLC hardware with 1000 bar / 15000 psi pressure limit

     

     

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    Floor 13, No.179 of North TianHe Road, GuangZhou, 510620, China
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