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AVANTOR® ACE® EXCEL® OLIGO COLUMN OLIGONUCLEOTIDE ANALYSIS
? Oligonucleotides are being synthesised for therapeutic usage against many diseases including genetic disorders and cancer
? Several impurities can be produced during oligonucleotide synthesis (e.g.failure sequences) which need to be separated from the desired product
? Ion-pair RPLC can be employed for the analysis of trityl-off deprotectedoligonucleotides
? Triethylammonium acetate (TEAA) is used to ion pair with the negativelycharged phosphodiester backbone of the oligonucleotide
? The stationary phase is chemically modified to withstand an extended pH range

OLIGONUCLEOTIDE LADDER STANDARD
? The oligonucleotide ladder standard demonstrated in this brochure consists of six DNA oligonucleotides of varying sequence length from 15 to 40 mer
? Each oligonucleotide sequence is made up of several thymidine monomers joined together via phosphodiester linkages as shown below

PRODUCT SPECIFICATIONS

To extend column lifetime ACE UHPLC guard cartridges (EXL-1P15-GD2U / EXL-1P15-GD4U) are recommended. For the guard system, a guard cartridge holder (H0011) is also required.
For HPLC column connections up to 6000 psi, PEEK? fingertight fittings (ACE-CC10) are recommended - for further details please contact 020-22826668
For UHPLC column connections up to 25000 psi, reuseable fittings (EXL-CC10) are recommended - for further details please contact 020-22826668

GUARANTEED REPRODUCIBILITY
? The ACE Excel Oligo column provides excellent batch-to-batch reproducibility as shown below

BATCH-TO-BATCH REPRODUCIBILITY

Columns: ACE Excel 1.7 Oligo, 50x2.1 mm Cat. No.: EXL-1715-0502 Mobile phase A: 80 mM TEAA in H2O (pH 7.0) Mobile phase B: 80 mM TEAA in MeCN Gradient: 12 – 15% B in 10 min Flow rate: 0.2 ml/min Temperature: 60 °C Injection: 5 μl Detection: 260 nm Columns: ACE Excel 3 Oligo, 150x4.6 mm Cat. No.: EXL-1115-1546 Mobile phase A: 80 mM TEAA in H2O (pH 7.0) Mobile phase B: 80 mM TEAA in MeCN Gradient: 12 – 15% B in 30 min Flow rate: 1.0 ml/min Temperature: 60 °C Injection: 20 μl Detection: 260 nm

MASS-SPEC COMPATIBLE
The ACE Oligo column is compatible with typical MS method conditions for oligonucleotide analysis
HFIP is commonly used in the mobile phase and was used for the separation shown below, in combination with dibutylamine

Sample: Oligonucleotide ladder standard
Column: ACE Excel 1.7 Oligo, 100x2.1 mm
Cat. No.: EXL-1715-1002
Mobile phase A: 15 mM dibutylamine + 25 mM 1,1,1,3,3,3-hexafluoropropan-2-ol in H2O
Mobile phase B: 15 mM dibutylamine + 25 mM 1,1,1,3,3,3-hexafluoropropan-2-ol in MeOH
Gradient: 30 – 50% B in 15 min
Flow rate: 0.6 ml/min
Temperature: 60 °C
Injection: 10 μl
Detection: 260 nm

 

EXCELLENT COLUMN STABILITY
? The ACE Oligo column shows excellent stability for oligonucleotide analysis over 5000 column volumes as shown below

 

COLUMN STABILITY STUDY

Sample: Oligonucleotide ladder standard
Mobile phase A: 100 mM TEAA in H2O (pH 7.0)
Mobile phase B: 100 mM TEAA in MeCN (pH 7.0)
Gradient: 10 – 14% B in 10 min
Flow rate: 0.6 ml/min
Temperature: 65 °C
Injection: 5 μl
Detection: 260 nm

 

ORDERING INFORMATION
U/HPLC hardware with 1000 bar / 15000 psi pressure limit