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    • 【FLM EN】ACE HILIC Columns ACE NOVEL REVERSED-PHASE DESIGN
      Many of the newest ACE phases were developed because of shortcomings with existing phenyl, pentafluorophenyl, cyano,polar embedded and C18 columns on the market today. These problems include inadequate stability at low pH, unacceptable bleed (ligand cleavage), pore dewetting and poor peak shape for basic and acidic analytes. ACE's ability to design new stationary phase ligands is an effort to address these shortcomings.
    詳情

    ACE NOVEL REVERSED-PHASE DESIGN
    Many of the newest ACE phases were developed because of shortcomings with existing phenyl, pentafluorophenyl, cyano,  polar embedded and C18 columns on the market today. These problems include inadequate stability at low pH, unacceptable bleed (ligand cleavage), pore dewetting and poor peak shape for basic and acidic analytes. ACE's ability to design new stationary phase ligands is an effort to address these shortcomings.


    固定相

    描述

    ACE® C18
    OCTADECYL SILANE LIGAND


    ? Optimized for maximum efficiency, superior peak shape and resolution
    ? Utilizes ultra-high purity silica for excellent peak shape and reproducibility



    ACE® C18-AR
    PROPRIETARY EXTENDED
    STABILITY LIGAND

    ? A unique phase that has an extended alkyl chain with a terminal phenyl group
    ? Provides improved retention and stability compared to commercial phenylpropyl phases
    ? Usable in 100% aqueous eluents

    ACE® C18-PFP
    PROPRIETARY EXTENDED
    STABILITY LIGAND

    ? A unique phase that has an extended alkyl chain with a terminal pentafluorophenyl group
    ? Provides improved retention and stability compared to commercial pentafluorophenylpropyl phases
    ? Usable in 100% aqueous eluents

    ACE® SUPERC18
    PROPRIETARY ENCAPSULATED
    BONDING TECHNOLOGY

    ? A uniquely-bonded EBT? (Encapsulated Bonding Technology) end-capped C18 phase, which provides unprecedented inertness and unparalleled efficiency over an extended pH range (pH 1.5–11.5)
    ? Excellent choice for low, mid and high pH work

    ACE® C18-AMIDE
    PROPRIETARY EXTENDED
    STABILITY LIGAND

    ? A uniquely-designed polar-embedded phase, which offers enhanced retention and resolution of polar acidic, phenolic, and hydroxy-substituted analytes
    ? Usable in 100% aqueous eluents

    ACE® CN-ES
    PROPRIETARY EXTENDED
    STABILITY LIGAND

    ? A unique phase that has an extended alkyl chain with a terminal cyano group
    ? Provides C18-like retention and stability compared to commercial cyanopropyl phases
    ? Usable with 100% aqueous mobile phases in RPLC mode, and also has excellent performance in normal-phase mode (NPLC)


    ACE NOVEL HILIC PHASE DESIGN
    ACE HILIC columns provide alternative selectivity to ACE RPLC columns with excellent retention for polar analytes.

    ACE® HILIC-A
    PROPRIETARY ACIDIC PHASE

    ? An acidic character phase with an ionizable surface charge depending upon mobile phase pH

    ACE® HILIC-B
    PROPRIETARY BASIC PHASE

    ? A basic character phase with an ionizable positive surface charge depending upon mobile phase pH

    ACE® HILIC-N
    PROPRIETARY NEUTRAL PHASE

    ? A polar neutral character phase capable of H-bonding with other mechanisms of interaction



    ACE UHPLC HILIC COLUMNS



    What is HILIC?
    ? HILIC is ideal for the separation and retention of polar species including polar neutral and polar ionised analytes
    ? HILIC separations typically include a polar stationary phase with high organic solvent containing mobile phases
    ? Mechanistically HILIC is complex and provides multiple modes of interaction between the analyte, stationary phase,eluent and water enriched layer at the stationary phase particle-eluent interface





    When Should You Consider HILIC?
    ? HILIC provides the retention and separation of hydrophilic or polar to very polar analytes not well retained in RPLC
    ? Hydrophilic or polar to very polar analytes have log P values (measure of lipophilicity) of around zero or less
    ? Generally, polar analytes are suitable for HILIC if they elute before caffeine in gradient RPLC


    Note: At mid pH, longest column lifetime of silica and bonded silica columns is achieved by avoiding carbonate, phosphate and borate buffers, and by using concentrations in the 5-10 mM range with column temperatures at or below 40 °C.


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