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【FLM EN】ACE HILIC Columns
ACE NOVEL REVERSED-PHASE DESIGN
Many of the newest ACE phases were developed because of shortcomings with existing phenyl, pentafluorophenyl, cyano,polar embedded and C18 columns on the market today. These problems include inadequate stability at low pH, unacceptable bleed (ligand cleavage), pore dewetting and poor peak shape for basic and acidic analytes. ACE's ability to design new stationary phase ligands is an effort to address these shortcomings.
ACE NOVEL REVERSED-PHASE DESIGN
Many of the newest ACE phases were developed because of shortcomings with existing phenyl, pentafluorophenyl, cyano, polar embedded and C18 columns on the market today. These problems include inadequate stability at low pH, unacceptable bleed (ligand cleavage), pore dewetting and poor peak shape for basic and acidic analytes. ACE's ability to design new stationary phase ligands is an effort to address these shortcomings.
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固定相 |
描述 |
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ACE® C18 |
? Optimized for maximum efficiency, superior peak shape and resolution
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ACE® C18-AR PROPRIETARY EXTENDED STABILITY LIGAND |
? A unique phase that has an extended alkyl chain with a terminal phenyl group |
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ACE® C18-PFP PROPRIETARY EXTENDED STABILITY LIGAND |
? A unique phase that has an extended alkyl chain with a terminal pentafluorophenyl group |
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ACE® SUPERC18 PROPRIETARY ENCAPSULATED BONDING TECHNOLOGY |
? A uniquely-bonded EBT? (Encapsulated Bonding Technology) end-capped C18 phase, which provides unprecedented inertness and unparalleled efficiency over an extended pH range (pH 1.5–11.5) |
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ACE® C18-AMIDE PROPRIETARY EXTENDED STABILITY LIGAND |
? A uniquely-designed polar-embedded phase, which offers enhanced retention and resolution of polar acidic, phenolic, and hydroxy-substituted analytes |
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ACE® CN-ES PROPRIETARY EXTENDED STABILITY LIGAND |
? A unique phase that has an extended alkyl chain with a terminal cyano group |
ACE NOVEL HILIC PHASE DESIGN
ACE HILIC columns provide alternative selectivity to ACE RPLC columns with excellent retention for polar analytes.
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ACE® HILIC-A |
? An acidic character phase with an ionizable surface charge depending upon mobile phase pH |
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ACE® HILIC-B |
? A basic character phase with an ionizable positive surface charge depending upon mobile phase pH |
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ACE® HILIC-N PROPRIETARY NEUTRAL PHASE |
? A polar neutral character phase capable of H-bonding with other mechanisms of interaction |
ACE UHPLC HILIC COLUMNS
What is HILIC?
? HILIC is ideal for the separation and retention of polar species including polar neutral and polar ionised analytes
? HILIC separations typically include a polar stationary phase with high organic solvent containing mobile phases
? Mechanistically HILIC is complex and provides multiple modes of interaction between the analyte, stationary phase,eluent and water enriched layer at the stationary phase particle-eluent interface
When Should You Consider HILIC?
? HILIC provides the retention and separation of hydrophilic or polar to very polar analytes not well retained in RPLC
? Hydrophilic or polar to very polar analytes have log P values (measure of lipophilicity) of around zero or less
? Generally, polar analytes are suitable for HILIC if they elute before caffeine in gradient RPLC
Note: At mid pH, longest column lifetime of silica and bonded silica columns is achieved by avoiding carbonate, phosphate and borate buffers, and by using concentrations in the 5-10 mM range with column temperatures at or below 40 °C.
